| 產(chǎn)品編號 |
bs-1228R-Gold |
| 英文名稱1 |
Rabbit Anti-alpha Adaptin/Gold Conjugated antibody
|
| 中文名稱 |
膠體金標記的銜接蛋白α-Adaptin抗體 |
| 別 名 |
100 kDa coated vesicle protein A; 100 kDa coated vesicle protein C; adaptin alpha A; Adaptin alpha B; Adaptor protein complex AP 2 alpha 1 subunit; Adaptor protein complex AP 2 alpha 2 subunit; Adaptor related protein complex 2 alpha 1 subunit; Adaptor related protein complex 2 alpha 2 subunit; ADTAA; ADTAB; Alpha adaptin A; Alpha adaptin C; AP 2 complex subunit alpha 1; AP 2 complex subunit alpha 2; AP2A2; CLAPA1; CLAPA2; Clathrin assembly protein complex 2 alpha A large chain; Clathrin assembly protein complex 2 alpha C large chain; Clathrin associated/assembly/adaptor protein large alpha 1; Clathrin associated/assembly/adaptor protein large alpha 2; HIP9; Huntingtin interacting protein HYPJ; Huntingtin interacting protein J; HYPJ; KIAA0899; Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit; Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit; AP2A2_HUMAN; AP-2 complex subunit alpha-2; Adapter-related protein complex 2 alpha-2 subunit; Adaptor protein complex AP-2 subunit alpha-2; Alpha-adaptin C; Alpha2-adaptin; Clathrin assembly protein complex 2 alpha-C large chain; Huntingtin yeast partner J; Huntingtin-interacting protein 9; HIP-9; Huntingtin-interacting protein J; Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit. |
| 規(guī)格價格 |
100ul/2980元
購買 大包裝/詢價 |
| 說 明 書 |
100ul（10nm 15nm 35nm）
|
| 研究領(lǐng)域 |
細胞生物 表觀遺傳學 |
| 抗體來源 |
Rabbit |
| 克隆類型 |
Polyclonal |
| 交叉反應(yīng) |
Human, Mouse,
(predicted: Rat, Cow, Horse, Rabbit, )
|
| 產(chǎn)品應(yīng)用 |
IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 |
103kDa |
| 性 狀 |
Lyophilized or Liquid |
| 濃 度 |
0.4mg/ml |
| 免 疫 原 |
KLH conjugated synthetic peptide derived from human alpha Adaptin |
| 亞 型 |
IgG |
| 純化方法 |
affinity purified by Protein A |
| 儲 存 液 |
0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300. |
| 保存條件 |
Store at 2-8 oC for 3-6 months. Avoid repeated freeze/thaw cycles. |
| 產(chǎn)品介紹 |
background:
Clathrin-mediated endocytosis is the pathway by which many receptors for nutrients and hormones are internalized to be recycled or down-regulated. During formation of clathrin coated membranes, clathrin co-assembles with heterotetrameric molecules known as assembly polypeptides (APs) or adaptors which form a layer of protein coat between the clathrin lattice and the membrane. There are two characterized adaptors AP1 and AP2. AP1 is associated with clathrin coated vesicles at the trans-Golgi network and AP2 is associated with the endocytic clathrin coated vesicles at the plasma membrane and has been shown to specifically interact with Shc and EGF receptor. AP2 is composed of four subunits, two separate 100 kDa gene products with similar domain structures (alpha and beta adaptin) and a 50 and 17 kDa subunit. There are two alpha-adaptin genes, alpha A and alpha C which have a tissue specific pattern of expression.
Function:
Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.
Subunit:
Adaptor protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type subunit AP2A1 or AP2A2 and beta-type subunit AP2B1), a medium adaptin (mu-type subunit AP2M1) and a small adaptin (sigma-type subunit AP2S1). Binds EPN1, EPS15, AMPH, SNAP91 and BIN1. Interacts with HIP1. Interacts with DGKD isoform 2. Interacts with DENND1A, DENND1B and DENND1C. Interacts with FCHO1 and DAB2.
Subcellular Location:
Cell membrane. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Note=AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.
Similarity:
Belongs to the adaptor complexes large subunit family.
Database links:
Entrez Gene: 515216 Cow
Entrez Gene: 784636 Cow
Entrez Gene: 160 Human
Entrez Gene: 161 Human
Entrez Gene: 11771 Mouse
Entrez Gene: 11772 Mouse
Entrez Gene: 308578 Rat
Entrez Gene: 81637 Rat
Omim: 601026 Human
Omim: 607242 Human
SwissProt: Q0VCK5 Cow
SwissProt: O94973 Human
SwissProt: O95782 Human
SwissProt: P17426 Mouse
SwissProt: P17427 Mouse
SwissProt: P18484 Rat
Unigene: 2041 Cow
Unigene: 19121 Human
Unigene: 6877 Mouse
Unigene: 34928 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
adaptin α參與披網(wǎng)格蛋白小泡組裝的一種蛋白質(zhì), 在披網(wǎng)格蛋白小泡組裝中與受體的細胞質(zhì)結(jié)構(gòu)域相互作用, 起銜接作用.
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