| 產(chǎn)品編號 | bs-4188R-FITC |
| 英文名稱1 | Rabbit Anti-SGPL1/FITC Conjugated antibody |
| 中文名稱 | FITC標記的磷酸鞘氨醇裂解酶1抗體 |
| 別 名 | FLJ13811; hSPL; KIAA1252; S1P; S1PL; SGPL 1; SGPL1; SGPL1_HUMAN; SP lyase; SP-lyase 1; Sphingosine 1 phosphate aldolase; Sphingosine 1 phosphate lyase 1; Sphingosine-1-phosphate aldolase; Sphingosine-1-phosphate lyase 1; SPL 1; SPL. |
| 規(guī)格價格 | 50ug/1680元 購買 100ug/2980元 購買 大包裝/詢價 |
| 說 明 書 | 50ug 100ug |
| 研究領域 | 腫瘤 心血管 免疫學 信號轉(zhuǎn)導 脂蛋白 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Rat, (predicted: Mouse, Dog, Pig, Cow, Horse, Rabbit, ) |
| 產(chǎn)品應用 | Flow-Cyt=1ug/Test IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 63kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SGPL1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Elevates stress-induced ceramide production and apoptosis. Function: Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Elevates stress-induced ceramide production and apoptosis. Subcellular Location: Endoplasmic reticulum membrane; Single-pass type III membrane protein. Tissue Specificity: Found in liver and kidney. Similarity: Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily. Database links: Entrez Gene: 8879 Human Entrez Gene: 20397 Mouse SwissProt: O95470 Human SwissProt: Q8R0X7 Mouse Unigene: 499984 Human Unigene: 412319 Mouse Unigene: 431862 Mouse Unigene: 26953 Rat Omim: 603729 Human
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品圖片 |
Blank control: A549.
Primary Antibody (green line): Rabbit Anti-SGPL1/FITC Conjugated antibody (bs-4188R-FITC)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-FITC .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
