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Rabbit Anti-DDX5/p68 RNA Helicase/Biotin Conjugated antibody (bs-7117R-Bio)
訂購熱線:400-901-9800
訂購郵箱:sales@73327.net
訂購QQ:  400-901-9800
技術(shù)支持:techsupport@73327.net
說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價
產(chǎn)品編號 bs-7117R-Bio
英文名稱1 Rabbit Anti-DDX5/p68 RNA Helicase/Biotin Conjugated antibody
中文名稱 生物素標記的三磷酸腺苷依賴解旋酶ddx5抗體
別    名 ATP dependent RNA helicase DDX5; DDX 5; Ddx5; DDX5_HUMAN; DEAD (Asp Glu Ala Asp) box helicase 5; DEAD (Asp Glu Ala Asp) box polypeptide 5; DEAD box 5; DEAD box protein 5; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 5 (RNA helicase, 68kD); DEAD/H (Asp Glu Ala Asp/His) box polypeptide 5; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase 68kDa); DEAD/H box-5 (RNA helicase 68kD); DKFZp686J01190; G17P1; HELR; HLR 1; HLR1; HUMP68; P68; P68; p68 RNA helicase; Probable ATP dependent RNA helicase DDX5; Probable ATP-dependent RNA helicase DDX5; RNA helicase 68kD; RNA helicase p68; RNA-dependent ATPase.   
規(guī)格價格 100ul/2980元 購買        大包裝/詢價
說 明 書 100ul  
研究領(lǐng)域 發(fā)育生物學  染色質(zhì)和核信號  神經(jīng)生物學  信號轉(zhuǎn)導  激酶和磷酸酶  細胞分化  表觀遺傳學  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Mouse,  (predicted: Human, Rat, Chicken, Cow, Horse, Rabbit, )
產(chǎn)品應用 WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 69kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human DDX5/p68 RNA Helicase(312-345aa)
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
p68 RNA helicase is a nuclear protein that exhibits RNA-dependent ATPase activity. Phosphorylation by protein kinase C inhibits p68 RNA helicase activity. p68 RNA helicase appears to play a role in organ differentiation during development. Furthermore, p68 RNA helicase is expressed in early neural development and in various mesodermal tissues in a number of different chordate embryos. At the cellular level, the expression levels of p68 RNA helicase increases in serum-induced quiescent cell lines. p68 RNA helicase may function as a coactivator for estrogen receptor alpha. Additionally, p68 RNA helicase associates with transcriptional coactivators CBP and p300. p68 RNA helicase localizes to the nucleus under normal conditions. During late telophase, p68 RNA helicase and fibrillarin colocalize to nascent nucleoli. p68 RNA helicase may function as a heterodimer with p72 RNA helicase.

Function:
RNA-dependent ATPase activity. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. May be involved in pre-mRNA splicing.

Subunit:
Interacts with BRDT (By similarity). Identified in thespliceosome C complex. Interacts with RBM4; the interaction occursin a RNA-independent manner. Interacts with EIF2C1 and EIF2C2.Interacts with ESR1; the interaction is enhanced by phosphorylationof ESR1 AF-1 domain. Interacts with AR, NCOA1, NCOA2, NCOA3, EP300,CREBBP, POLR2A, TP53, RUNX2 and HDAC1. Self-associates. Interactswith DDX17. [INTERACTION] Q12873:CHD3; NbExp=4; IntAct=EBI-351962, EBI-523590;P45481:Crebbp (xeno); NbExp=3; IntAct=EBI-351962, EBI-296306;Q09472:EP300; NbExp=4; IntAct=EBI-351962, EBI-447295; P03372:ESR1;NbExp=8; IntAct=EBI-351962, EBI-78473; P22087:FBL; NbExp=6;IntAct=EBI-351962, EBI-358318; Q13547:HDAC1; NbExp=4;IntAct=EBI-351962, EBI-301834; O95983:MBD3; NbExp=4;IntAct=EBI-351962, EBI-1783068; P10085:Myod1 (xeno); NbExp=3;IntAct=EBI-351962, EBI-4405734; P24928:POLR2A; NbExp=3;IntAct=EBI-351962, EBI-295301; Q08775-3:Runx2 (xeno); NbExp=2;IntAct=EBI-351962, EBI-6119991; P04637:TP53; NbExp=3;IntAct=EBI-351962, EBI-366083; P04637-1:TP53; NbExp=2;IntAct=EBI-351962, EBI-3895849; P04637-7:TP53; NbExp=2;IntAct=EBI-351962, EBI-3895873.

Subcellular Location:
Nucleus

Post-translational modifications:
Arg-502 is dimethylated, probably to asymmetricdimethylarginine.
Sumoylated; sumoylation, promoted by PIAS1, promotesinteraction with HDAC1 and transcriptional repression activity.Sumoylation also significantly increases stability, and reducespolyubiquitination.
Polyubiquitinated, leading to proteasomal degradation.

Similarity:
Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.

Database links:

Entrez Gene: 1655 Human

Entrez Gene: 13207 Mouse

Entrez Gene: 287765 Rat

Omim: 180630 Human

SwissProt: P17844 Human

SwissProt: Q61656 Mouse

Unigene: 279806 Human

Unigene: 220038 Mouse

Unigene: 162208 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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