| 產(chǎn)品編號 | bs-10050R-HRP |
| 英文名稱1 | Rabbit Anti-IBDV/HRP Conjugated antibody |
| 中文名稱 | 辣根過氧化物酶標記的雞傳染性法氏囊病病毒抗體 |
| 別 名 | Infectious Bursal Disease Vaccine; Infectious Bursal Disease virus; infectious bursal disease; IBD. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 免疫學(xué) 細菌及病毒 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | |
| 產(chǎn)品應(yīng)用 | ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Infectious Bursal Disease Vaccine |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Infectious bursal disease (also known as IBD, Gumboro Disease, Infectious Bursitis and Infectious Avian Nephrosis) is a highly contagious disease of young chickens caused by infectious bursal disease virus (IBDV), characterized by immunosuppression and mortality generally at 3 to 6 weeks of age. IBDV is a double stranded RNA virus that has a bi-segmented genome and belongs to the genus Avibirnavirus of family Birnaviridae. There are two distinct serotypes of the virus, but only serotype 1 viruses cause disease in poultry. At least six antigenic subtypes of IBDV serotype 1 have been identified by in vitro cross-neutralization assay. Viruses belonging to one of these antigenic subtypes are commonly known as variants, which were reported to break through high levels of maternal antibodies in commercial flocks, causing up to 60 to 100 percent mortality rates in chickens. With the advent of highly sensitive molecular techniques, such as reverse transcription polymerase chain reaction (RT-PCR) and restriction fragment length polymorphism (RFLP), it became possible to detect the vvIBDV, to differentiate IBDV strains, and to use such information in studying the molecular epidemiology of the virus. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 由雙鏈RNA病毒科的傳染性法氏囊病病毒(IBDV)所引起的一種雞的急性暴發(fā)性傳染病。表現(xiàn)為法氏囊淋巴組織和淋巴細胞壞死，發(fā)病率及死亡率均高。耐過雞的法氏囊組織受損，導(dǎo)致免疫抑制，影響其他疫苗的免疫效果，常造成免疫失敗。 IBDV的自然宿主僅為雛雞和火雞。從雞分離的IBDV只感染雞，感染火雞不發(fā)病，但能引起抗體產(chǎn)生。同樣，從火雞分離的病毒僅能使火雞感染，而不感染雞。不同品種的雞均有易感性。IBD母源抗體陰性的雞可于1周齡內(nèi)感染發(fā)病，有母源抗體的雞多在母源抗體下降至較低水平時感染發(fā)病。3～6周齡的雞最易感。也有15周齡以上雞發(fā)病的報道。該病全年均可發(fā)生，無明顯季節(jié)性。 病雞的糞便中含有大量病毒，病雞是主要傳染源。雞可通過直接接觸和污染了IBDV的飼料、飲水、墊料、塵埃、用具、車輛、人員、衣物等間接傳播，老鼠和甲蟲等也可間接傳播。有人從蚊子體內(nèi)分離出一株病毒，被認為是一株IBDV自然弱毒，由此說明媒介昆蟲可能參與該病的傳播。該病毒不僅可通過消化道和呼吸道感染，還可通過污染了病毒的蛋殼傳播，但未有證據(jù)表明經(jīng)卵傳播。另外，經(jīng)眼結(jié)膜也可傳播。 |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
