| 產(chǎn)品編號(hào) | bs-13663R-BF488 |
| 英文名稱1 | Rabbit Anti-phospho-SLP76 (Tyr113)/BF488 Conjugated antibody |
| 中文名稱 | BF488標(biāo)記的磷酸化淋巴細(xì)胞胞漿蛋白2抗體 |
| 別 名 | SLP76 (phospho Y113); p-SLP76 (Y113); p-LCP2; 76 kDa tyrosine phosphoprotein; CG8697; LCP 2; LCP 2; LCP2; LCP2_HUMAN; Lymphocyte Cytosolic Protein 2; Lymphocyte Cytosolic Protein 2; SH2 Domain Containing Leukocyte Protein 76 KD; SH2 domain containing leukocyte protein of 76kD; SH2 domain containing leukocyte protein of 76kDa; SH2 domain-containing leukocyte protein of 76 kDa; SLP 76; SLP 76; SLP 76 tyrosine phosphoprotein; SLP-76 tyrosine phosphoprotein; SLP76; SLP76 tyrosine phosphoprotein. |
| 規(guī)格價(jià)格 | 100ul/2980元 購買 大包裝/詢價(jià) |
| 說 明 書 | 100ul |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 激酶和磷酸酶 淋巴細(xì)胞 t-淋巴細(xì)胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Human, Pig, Cow, Horse, Rabbit, Sheep, ) |
| 產(chǎn)品應(yīng)用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 75kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human SLP76 around the phosphorylation site of Tyr113 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: The translational product of the Vav proto-oncogene is exclusively expressed in cells of hematopoietic origin and is critical for lymphocyte development and activation. However, the biochemical basis of Vav’s function is unclear. Vav contains a single SH2 domain that is required for its association with the T cell receptor (TCR). Overexpression of Vav or SLP-76 in Jurkat cells leads to NFAT activation and IL-2 production. When co-expressed, Vav and SLP-76 synergize to induce a robust basal and TCR-mediated IL-2 response. Although SLP-76 does not contain a motif that would indicate it to be a member of the tyrosine, serine/threonine or lipid kinase families, it does contain several putative SH2/SH3-binding domains and has been shown to physically associate with the adapter protein GRB2 as well as PLC g1. The discovery of SLP-76 represents an important step in elucidating the mechanism of Vav transformation and TCR-mediated NFAT activation. Function: Involved in T-cell antigen receptor mediated signaling. Subunit: Interacts with SLA. Interacts with CBLB (By similarity). Interacts with the adapter proteins GRB2 and FYB. Interacts with SHB. Interacts with PRAM1. Subcellular Location: Cytoplasm. Tissue Specificity: Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines. Post-translational modifications : Phosphorylated after T-cell receptor activation by ZAP-70. Similarity: Contains 1 SAM (sterile alpha motif) domain. Contains 1 SH2 domain. Database links: Entrez Gene: 3937 Human Omim: 601603 Human SwissProt: Q13094 Human Unigene: 304475 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
