| 產(chǎn)品編號 | bs-17192R-BF647 |
| 英文名稱1 | Rabbit Anti-IZUMO1/BF647 Conjugated antibody |
| 中文名稱 | BF647標記的IZUMO1蛋白抗體 |
| 別 名 | IZUM1_HUMAN; IZUMO; Izumo sperm egg fusion 1; Izumo sperm-egg fusion protein 1; Izumo1; MGC34799; OBF; Oocyte binding/fusion factor; Sperm specific protein izumo; Sperm-specific protein izumo. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 細胞生物 發(fā)育生物學(xué) 細胞分化 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Human, Mouse, Rat, ) |
| 產(chǎn)品應(yīng)用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 39kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IZUMO1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: The sperm-specific protein Izumo, named for a Japanese shrine dedicated to marriage, is essential for sperm-egg plasma membrane binding and fusion (Inoue et al., 2005 [PubMed 15759005]).[supplied by OMIM, Mar 2008] Function: Involved in the fertilization process. May be involved in the fusion of the sperm with the egg. Subunit: Monomer, homodimer and homooligomer. Interacts with IZUMO1R/JUNO (By similarity). Subcellular Location: Membrane. Tissue Specificity: Sperm-specific (at protein level). Detectable on sperm surface only after the acrosome reaction. Post-translational modifications: N-glycosylated. Glycosylation is not essential for fusion and for proper protein trafficking in sperm. Phosphorylated. Similarity: Belongs to the Izumo family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Database links: Entrez Gene: 284359 Human Entrez Gene: 73456 Mouse Omim: 609278 Human SwissProt: Q8IYV9 Human SwissProt: Q9D9J7 Mouse Unigene: 400688 Human Unigene: 380445 Mouse Unigene: 125416 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
