| 產(chǎn)品編號 |
bsm-33331M-PE-Cy5 |
| 英文名稱1 |
Mouse Anti-phospho-MLKL (Ser358)/PE-Cy5 Conjugated antibody
|
| 中文名稱 |
PE-Cy5標(biāo)記的磷酸化MLKL單克隆抗體 |
| 別 名 |
phospho-MLKL(S358); p-MLKL(S358); MLKL(p-S358);9130019I15Rik; FLJ34389; hMLKL; Mixed lineage kinase domain like; Mixed lineage kinase domain like protein; Mixed lineage kinase domain-like protein; MLKL_HUMAN. |
| 規(guī)格價(jià)格 |
100ul/2980元
購買 大包裝/詢價(jià) |
| 說 明 書 |
100ul
|
| 研究領(lǐng)域 |
細(xì)胞生物 激酶和磷酸酶 |
| 抗體來源 |
Mouse |
| 克隆類型 |
Monoclonal |
| 克 隆 號 |
8C4 |
| 交叉反應(yīng) |
|
| 產(chǎn)品應(yīng)用 |
ICC=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 |
54kDa |
| 性 狀 |
Lyophilized or Liquid |
| 濃 度 |
1mg/ml |
| 免 疫 原 |
KLH conjugated synthetic peptide derived from human phospho-MLKL (Ser358) |
| 亞 型 |
IgG |
| 純化方法 |
affinity purified by Protein G |
| 儲 存 液 |
Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.05M PB, pH 7.5. |
| 保存條件 |
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background:
This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015].
Function:
Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity.
Subunit:
Homotrimer; forms homotrimers on necroptosis induction. Interacts with RIPK3; the interaction is direct. Upon TNF-induced necrosis, forms in complex with PGAM5, RIPK1 and RIPK3. Within this complex, may play a role in the proper targeting of RIPK1/RIPK3 to its downstream effector PGAM5.
Subcellular Location:
Cytoplasm. Cell membrane. Note=Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction.
Post-translational modifications:
Phosphorylation by RIPK3 induces a conformational switch that is required for necroptosis. It also induces homotrimerization and localization to the plasma membrane.
Similarity:
Belongs to the protein kinase superfamily.
Contains 1 protein kinase domain.
Database links:
Entrez Gene: 197259 Human
Entrez Gene: 74568 Mouse
Entrez Gene: 690743 Rat
Omim: 615153 Human
SwissProt: Q8NB16 Human
SwissProt: Q9D2Y4 Mouse
Unigene: 119878 Human
Unigene: 207971 Mouse
Unigene: 105677 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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